Human antigen R (HuR) is an RNA-binding protein that regulates the stability, translation, and nucleus-to-cytoplasm shuttling of its target mRNAs. While HuR is normally localized with …. The stability of mRNA is one of the key factors governing the regulation of eukaryotic gene expression and function. Human antigen R (HuR) is an RNA-binding
Aug 5, 2011 Upon HuR knockdown, mRNA levels and protein synthesis of thousands of The Hu/ELAV family of RNA binding proteins is conserved across
Recent studies showed that HuR is related to autophagy 26; also, HuR is regarded as a crucial metabolic regulator of diabetes,27 which implied that HuR may play The 3′ UTR of p53 was found to be a target of the RNA-binding protein HuR in a UVC-dependent manner in vitro and in vivo. HuR-overexpressing RKO cells displayed elevated p53 levels, whereas cells expressing reduced HuR showed markedly diminished p53 abundance and p53 translation. RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462, PubMed:31358969). Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity).
Human antigen R (HuR) is a member of the Hu family of RNA-binding proteins. This molecule, which was first described in tumors nearly two decades ago, has recently received much attention in tumor-related research because it regulates the expression of many tumor-associated molecules through posttranscriptional regulatory mechanisms, thereby affecting biological characteristics. The RNA-binding protein HuR post-transcriptionally regulates mRNA splicing. Turner and colleagues demonstrate that HuR serves a critical role in thymus-independent antigen responses by regulating Protein 1, ELAVL1) is an RNA-binding protein,26 it may effect the stabilization of mRNAs so as to regulate their expression and is in-volved in various biological progresses. Recent studies showed that HuR is related to autophagy 26; also, HuR is regarded as a crucial metabolic regulator of diabetes,27 which implied that HuR may play The 3′ UTR of p53 was found to be a target of the RNA-binding protein HuR in a UVC-dependent manner in vitro and in vivo.
It can shuttle between the nucleus and the cytoplasm, and the stabilization of ARE-mRNA by HuR is believed to be linked to its localization in the cytoplasm (12,13).
Hur vet RNA-polymeras var den ska starta transkriptionen i en bakterie? (transkriptionsstartpunkten) Innehåller TBP (tata-binding protein) och TAFs (TBP). 24
Here, we investigate the potential contribution of the RNA binding protein (RBP) human antigen R (HuR) on the posttranscriptional regulation of ABCA1 expression HuR/ELAVL1 is an RNA-binding protein involved in differentiation and stress response that acts primarily by stabilizing messenger RNA (mRNA) targets. HuR comprises three RNA recognition motifs (RRMs) where the structure and RNA binding of RRM3 and of full-length HuR remain poorly understood.
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Here we show that HuR is required for the efficacy of chemotherapies in HuR is an RNA-binding protein that resides predominantly in the nucleus but shuttles to the cytosol carrying mRNAs containing a uridylate-rich region in the 3'untranslated region.
ubiquitously expressed mRNA-binding protein (mRBP) HuR(ELAVL1)isapostulatedstress-relevantproteinthat binds to adenylate uridylate (AU)–rich sites within regu-latory mRNA targets and regulates their expression via post-transcriptional mechanisms (12). These binding sites typically reside within 39–UTRs of target transcripts. HuR, a protein that binds to specific mRNA subsets, is increasingly recognized as a pivotal posttranscriptional regulator of gene expression. Here, HuR was immunoprecipitated under conditions that preserved HuR-RNA interactions, and HuR-bound target mRNAs were identified by cDNA array hybridization. PDCD4 mRNA translation is regulated by an interplay between the oncogenic microRNA miR-21 and the RNA-binding protein (RBP) HuR in response to LPS stimulation, but the role of other regulatory factors remain unknown.
Hattrick
A scrambled sequence was utilized to generate control (shControl) clones. Effective and specific knockdown of HuR was observed at the mRNA and protein levels (Supplementary Fig. S1). Human antigen R (HuR) is an RNA-binding protein that posttranscriptionally regulates many cancer-trait genes.
In the nucleus, HuR is involved in various functions, including RNA splicing and nuclear export. HuR (Human antigen R, encoded by Embryonic Lethal Abnormal Vision‐Like Protein 1, ELAVL1) is an RNA‐binding protein, 26 it may effect the stabilization of mRNAs so as to regulate their expression and is involved in various biological progresses. 2020-01-10 · Human antigen R (HuR) is an RNA binding protein that promotes mRNA stability. Here the authors show that HuR represses adipogenesis in white and brown adipose tissue by stabilizing Insig1 and
ELAV-like protein 1 or HuR is a protein that in humans is encoded by the ELAVL1 gene.
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This Custom Cell Based Screening Kit contains reagents necessary to create a complete HR reporter system in any animal cell line.
These binding sites typically reside within 3‘‐UTRs of target transcripts. 2012-06-01 · The mammalian RNA-binding protein (RBP) HuR associates with numerous mRNAs encoding proteins with roles in cell division, cell survival, immune response, and differentiation. HuR was known to stabilize many of these mRNAs and/or modulated their translation, but the molecular processes by which HuR affected the fate of target mRNAs was largely unknown. The mammalian RNA-binding protein (RBP) HuR associates with numerous mRNAs encoding proteins with roles in cell division, cell survival, immune response, and differentiation. HuR was known to stabilize many of these mRNAs and/or modulated their translation, but the molecular processes by which HuR affected the fate of target mRNAs was largely unknown. The impact of HuR binding to bcl-2 mRNA was determined by HuR knockdown. We generated 2 stable U251 clones expressing short hairpin (sh) RNA directed to HuR (shHuR).